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Analysis of castor bean ribosome-inactivating proteins and their gene expression during seed development Genet. Mol. Biol.
Loss-Morais,Guilherme; Turchetto-Zolet,Andreia Carina; Etges,Matheus; Cagliari,Alexandro; Körbes,Ana Paula; Maraschin,Felipe dos Santos; Margis-Pinheiro,Márcia; Margis,Rogério.
Ribosome-inactivating proteins (RIPs) are enzymes that inhibit protein synthesis after depurination of a specific adenine in rRNA. The RIP family members are classified as type I RIPs that contain an RNA-N-glycosidase domain and type II RIPs that contain a lectin domain (B chain) in addition to the glycosidase domain (A chain). In this work, we identified 30 new plant RIPs and characterized 18 Ricinus communis RIPs. Phylogenetic and functional divergence analyses indicated that the emergence of type I and II RIPs probably occurred before the monocot/eudicot split. We also report the expression profiles of 18 castor bean genes, including those for ricin and agglutinin, in five seed stages as assessed by quantitative PCR. Ricin and agglutinin were the most...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agglutinin; Evolution; Lipase; Ricinus communis; Ricin; RIPs.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100011
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Karyotipic asymmetry of both wild and cultivated species of Pennisetum Bragantia
Techio,Vânia Helena; Davide,Lisete Chamma; Cagliari,Alexandro; Barbosa,Sandro; Pereira,Antônio Vander.
This study aimed the establishment of the relation between karyotipic asymmetry values obtained for different accessions of both wild and cultivated species of Pennisetum from Germplasm Bank of Embrapa Gado de Leite/Juiz de Fora-Minas Gerais State, Brazil. Conventional cell cycle synchronization protocols and Feulgen staining method were used to obtain metaphases plates. The wild-type accessions corresponded to the species P. setosum (2n=6x=54), P. nervosum (2n=4x=36), and P. orientale (2n=4x=36), and the cultivated to P. purpureum (2n=4x=28) and P. glaucum (2n=2x=14). No significant difference was found for the total length of chromosomes (p>0.05) among the species. The analysis of intra-chromosomal asymmetry (A1) and inter-chromosomal asymmetry (A2)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pennisetum purpureum; Pennisetum glaucum; Germplasm; Karyotype.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052010000200003
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